Persistence of Trichinella spiralis in Rat Carcasses Experimentally Mixed in Different Feed

Trichinella spiralis infected rat carcasses were incubated for 6 weeks in several animal feeds to assess how long Trichinella can present a risk for an outbreak in contaminated feeds. In groups of 6, 24 infected target rats were placed in silage, grained barley, propionic acid-preserved feed, and also into simulated pasture conditions. Test environments were sampled after one-, 2-, 4-, and 6-week-incubations. Trichinella larvae were recovered by digestion, and their infectivity was evaluated in rats. A two-week incubation reduced the number of recovered larvae, but still after 6 weeks low numbers were isolated from all feeds except from the experimental group simulating pasture conditions. After 2 weeks storage, the larvae were infective in all storage environments. However, up to 4 weeks, they survived only in the propionic acid-fermented feed and there in small numbers with reduced reproductive capability. This indicates the possibility of farm animals to get infection from rats or other infected material being hazardously mixed with hay or other feed. If silage is stored for at least one month before use, however, the risk from this forage appears to be minimized

Software business : A short history and trends for the future

During its 70 years of existence, the software business has been following an evolution curve that can be considered typical for several fields of industrial businesses. Technological breakthroughs and innovations are typically seen as enablers for business evolution in the domain of technology and innovation management. Software, data collection, and data analysis represent a greater and greater part of the value of products and services, and today, their role is also becoming essential in more traditional fields. This, however, requires business and technology competences that traditional industries do not have. The transformation also enables new ways of doing business and opens the field for new kinds of players. Together, all this leads to transformation and new possibilities for the software industry. In this paper we study the overall trajectory of the software business, and then offer some viewpoints on the change in different elements of business models. Copyright © by the paper's authors. Copying permitted only for private and academic purposes.Peer reviewe

Predilection Muscles and Physical Condition of Raccoon Dogs (Nyctereutes procyonoides) Experimentally Infected with Trichinella spiralis and Trichinella nativa

The predilection muscles of Trichinella spiralis and T. nativa were studied in 2 experimental groups of 6 raccoon dogs (Nyctereutes procyonoides), the third group serving as a control for clinical signs. The infection dose for both parasites was 1 larva/g body weight. After 12 weeks, the animals were euthanized and 13 sampling sites were analysed by the digestion method. Larvae were found in all sampled skeleton muscles of the infected animals, but not in the specimens from the heart or intestinal musculature. Both parasite species reproduced equally well in the raccoon dog. The median density of infection in positive tissues was 353 larvae per gram (lpg) with T. spiralis and 343 lpg with T. nativa. All the infected animals had the highest larvae numbers in the carpal flexors (M. flexor carpi ulnaris). Also tongue and eye muscles had high infection levels. There were no significant differences in the predilection sites between these 2 parasite species. Trichinellosis increased the relative amount of fat, but not the body weight in the captive raccoon dogs. Thus, Trichinella as a muscle parasite might have catabolic effect on these animals

x509-free access to WLCG resources

Access to WLCG resources is authenticated using an x509 and PKI infrastructure. Even though HEP users have always been exposed to certificates directly, the development of modern Web Applications by the LHC experiments calls for simplified authentication processes keeping the underlying software unmodified. In this work we will show a solution with the goal of providing access to WLCG resources using the user's home organisations credentials, without the need for user-acquired x509 certificates. In particular, we focus on identity providers within eduGAIN, which interconnects research and education organisations worldwide, and enables the trustworthy exchange of identity-related information. eduGAIN has been integrated at CERN in the SSO infrastructure so that users can authenticate without the need of a CERN account. This solution achieves x509-free access to Grid resources with the help of two services: STS and an online CA. The STS (Security Token Service) allows credential translation from the SAML2 format used by Identity Federations to the VOMS-enabled x509 used by most of the Grid. The IOTA CA (Identifier-Only Trust Assurance Certification Authority) is responsible for the automatic issuing of short-lived x509 certificates. The IOTA CA deployed at CERN has been accepted by EUGridPMA as the CERN LCG IOTA CA, included in the IGTF trust anchor distribution and installed by the sites in WLCG. We will also describe the first pilot projects which are integrating the solution.Peer reviewe

Determining bacterial and host contributions to the human salivary metabolome

BACKGROUND: Salivary metabolomics is rapidly advancing. AIM AND METHODS: To determine the extent to which salivary metabolites reflects host or microbial metabolic activity whole-mouth saliva (WMS), parotid saliva (PS) and plasma collected contemporaneously from healthy volunteers were analysed by (1)H-NMR spectroscopy. Spectra underwent principal component analysis and k-means cluster analysis and metabolite quantification. WMS samples were cultured on both sucrose and peptide-enriched media. Correlation between metabolite concentration and bacterial load was assessed. RESULTS: WMS contained abundant short-chain fatty acids (SCFAs), which were minimal in PS and plasma. WMS spectral exhibited greater inter-individual variation than those of PS or plasma (6.7 and 3.6 fold, respectively), likely reflecting diversity of microbial metabolomes. WMS bacterial load correlated strongly with SCFA levels. Additional WMS metabolites including amines, amino acids and organic acids were positively correlated with bacterial load. Lactate, urea and citrate appeared to enter WMS via PS and the circulation. Urea correlated inversely with WMS bacterial load. CONCLUSIONS: Oral microbiota contribute significantly to the WMS metabolome. Several WMS metabolites (lactate, urea and citrate) are derived from the host circulation. WMS may be particularly useful to aid diagnosis of conditions reflective of dysbiosis. WMS could also complement other gastrointestinal fluids in future metabolomic studies

Changes in the household structure of the Finnish elderly by age, sex and educational attainment in 1987–2035

While the growth of the elderly population is both inevitable and predictable, the experience of old age is changing rapidly. Changes in living arrangements and family forms as well as cohorts’ changing experiences of socioeconomic environments over the life-course will shape the experience of old age and have a substantial impact on the well-being, health and care needs of the elderly population. According to the latest population projections by Statistics Finland, the number of older Finns (65 and older) and old-age dependency ratio are likely to increase particularly rapidly in the next 20 years or so. However, little is known about the future prospects of living arrangements of older Finns. We use an 11% longitudinal sample of Finns aged 40 years and older drawn from population registration data to evaluate changes in living arrangements of 65+ Finnish men and women from 1987 to 2011 and project living arrangements to 2035 by educational level. We estimate proportions in various living arrangements and calculate multistate life table estimates of years lived in particular states. Projections are based on dynamic transition probability forecasts in LIPRO with constant and variable rates. We show that living alone at older ages is currently more common among women than men: about 40% and 60% of women aged 65–79 and 80+ respectively, and about 20–30% of corresponding men live alone. These proportions are likely to start to decline slowly among women but increase among men under 80. Other living arrangements are becoming increasingly rare. Because of major changes in the access to further education the number of basic educated older people is declining rapidly. Educational differences in living arrangements are small among women, but among men living with a partner has been more common among the higher educated, although this advantage is decreasing. Of the remaining life expectancy at age 65 in 1987 women could expect to live about 40% with a partner; with the proportion increasing slightly to 2011 and 2035. Among men, these proportions were much higher in 1987 in all educational groups, but have declined slowly among the basic and secondary educated and quite markedly among the tertiary educated. Conversely, the much longer life expectancy of living alone among women as compared to men has narrowed somewhat. Overall, we know that the future elderly population will be better educated than ever before and is more likely to live with a spouse or partner. Future living arrangement distributions of older people are strongly determined, in particular, by past household behavior and, to a lesser extent, future changes in mortality. If life expectancy differences between men and women continue to converge in the long run, the proportion of remaining years of life spent living with a partner will increase among women and living alone will increase among men. However, it remains to be seen whether the better educated and partnered future elderly will benefit from the same social, functioning, health and mortality advantages as the well-educated elderly and partnered of today. In the past 25 years, some of these differences have remained sur-prisingly persistent; for example, despite large distributional changes in these characteristics, the strong health benefits of education and living with a spouse remain. Thus, if the past is a guide for the future, we may expect to see a better functioning elderly population as a consequence of these demographic changes

Changes in the production rate of secondary aerosol particles in Central Europe in view of decreasing SO2 emissions between 1996 and 2006

In anthropogenically influenced atmospheres, sulphur dioxide (SO2) is the main precursor of gaseous sulphuric acid (H2SO4), which in turn is a main precursor for atmospheric particle nucleation. As a result of socio-economic changes, East Germany has seen a dramatic decrease in anthropogenic SO2 emissions between 1989 and present, as documented by routine air quality measurements in many locations. We have attempted to evaluate the influence of changing SO2 concentrations on the frequency and intensity of new particle formation (NPF) using two different data sets (1996–1997; 2003–2006) of experimental particle number size distributions (diameter range 3–750 nm) from the atmospheric research station Melpitz near Leipzig, Germany. Between the two periods SO2 concentrations decreased by 65% on average, while the frequency of NPF events dropped by 45%. Meanwhile, the average formation rate of 3 nm particles decreased by 68% on average. The trends were statistically significant and therefore suggest a connection between the availability of anthropogenic SO2 and freshly formed new particles. In contrast to the decrease in new particle formation, we found an increase in the mean growth rate of freshly nucleated particles (+22%), suggesting that particle nucleation and subsequent growth into larger sizes are delineated with respect to their precursor species. Using three basic parameters, the condensation sink for H2SO4, the SO2 concentration, and the global radiation intensity, we were able to define the characteristic range of atmospheric conditions under which particle formation events take place at the Melpitz site. While the decrease in the concentrations and formation rates of the new particles was rather evident, no similar decrease was found with respect to the generation of cloud condensation nuclei (CCN; particle diameter >100 nm) as a result of atmospheric nucleation events. On the contrary, the production of CCN following nucleation events appears to have increased by tens of percents. Our aerosol dynamics model simulations suggest that such an increase can be caused by the increased particle growth rate

Culturally and linguistically diverse nursing students' experiences of integration into the working environment: A qualitative study

Background Understanding how culturally and linguistically diverse students experience clinical practice and their competence development is important to retaining registered nurses. This study aimed to describe culturally and linguistically diverse students' experiences of clinical practice, perceptions of their career path, and intentions to stay in the nursing profession. Methods A descriptive qualitative study was conducted. The participants were culturally and linguistically diverse nursing students (n = 22) from six Finnish higher education institutions. Nine focus-group interviews, with up to six students per group, were conducted during the spring and summer of 2021. Data were analysed using inductive content analysis. Results The factors which affected culturally and linguistically diverse students' intentions to stay in nursing profession in Finland consisted of support during university studies and clinical practice, perceived equality, nursing competence development, successful integration into the workplace and social life, and clinical practice experiences. Conclusions The results support the development of a model for culturally and linguistically diverse nurses' integration into the Finnish health care settings by identifying the key factors for an effective transition to the profession

EMI Security Architecture

This document describes the various architectures of the three middlewares that comprise the EMI software stack. It also outlines the common efforts in the security area that allow interoperability between these middlewares. The assessment of the EMI Security presented in this document was performed internally by members of the Security Area of the EMI project

A statistical proxy for sulphuric acid concentration

Gaseous sulphuric acid is a key precursor for new particle formation in the atmosphere. Previous experimental studies have confirmed a strong correlation between the number concentrations of freshly formed particles and the ambient concentrations of sulphuric acid. This study evaluates a body of experimental gas phase sulphuric acid concentrations, as measured by Chemical Ionization Mass Spectrometry (CIMS) during six intensive measurement campaigns and one long-term observational period. The campaign datasets were measured in Hyytiälä, Finland, in 2003 and 2007, in San Pietro Capofiume, Italy, in 2009, in Melpitz, Germany, in 2008, in Atlanta, Georgia, USA, in 2002, and in Niwot Ridge, Colorado, USA, in 2007. The long term data were obtained in Hohenpeissenberg, Germany, during 1998 to 2000. The measured time series were used to construct proximity measures ("proxies") for sulphuric acid concentration by using statistical analysis methods. The objective of this study is to find a proxy for sulfuric acid that is valid in as many different atmospheric environments as possible. Our most accurate and universal formulation of the sulphuric acid concentration proxy uses global solar radiation, SO2 concentration, condensation sink and relative humidity as predictor variables, yielding a correlation measure (R) of 0.87 between observed concentration and the proxy predictions. Interestingly, the role of the condensation sink in the proxy was only minor, since similarly accurate proxies could be constructed with global solar radiation and SO2 concentration alone. This could be attributed to SO2 being an indicator for anthropogenic pollution, including particulate and gaseous emissions which represent sinks for the OH radical that, in turn, is needed for the formation of sulphuric acid